5/25/2023 0 Comments India ink staining![]() Be cautious while handling the slide, since the organisms have not been killed.Begin by placing a euthanized mouse ventral side up on a surgical bed and securing the limbs of the animal.Dispose of staining waste and slides in designated waste containers.Clean your microscope with lens cleaner, removing all oil from lenses.You may need to decrease the amount of light in order to make the capsule easier to see. Results: Look for purple cells surrounded by a clear or faint blue halo on transparent background. DO NOT RINSE WITH WATER! Water will remove the capsule from the cell. Tilt the slide and rinse with 20% copper sulfate solution.This is done by contacting the drop of crystal violet with the clean microscope slide and using the capillary action of the dye/ slide to spread the crystal violet across the smear. Place the end of another clean microscope slide at an angle to the end of the slide containing the organism. ![]() If you are adding bacteria from a culture plate make sure that there are no large clumps of the organism, but try to avoid spreading the drop.
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